Table 2.
Antibodies used for flow cytometry analysis of peripheral blood mature lineages
| Marker | Fluorophore | Per 100 μl | Clone | Manufacturer | Catalog # |
|---|---|---|---|---|---|
| CD45.1 | PacBlue | 0.5 | A20 | BioLegend | 110722 |
| CD45.2 | APC | 0.3 | 104 | BioLegend | 109814 |
| B220 (CD45R) | FITC | 0.3 | RA3-6B2 | BioLegend | 103206 |
| CD4 | FITC | 0.5 | GK1.5 | BioLegend | 100406 |
| CD8 | FITC | 0.5 | 53-6.7 | BioLegend | 100706 |
| CD3 | FITC | 0.3 | 17A2 | BioLegend | 100706 |
| C-KIT (CD117) | PE | 0.5 | 2B8 | BioLegend | 105808 |
| B220 (CD45R) | APC-Cy7 | 0.3 | RA3-6B2 | BioLegend | 103224 |
| CD11B | APC-Cy7 | 0.1 | M1/70 | BioLegend | 101226 |