Reagent	Final concentration	Quantity
1 M Tris-HCl, pH 7.4	40 mM	2 mL
2 M KCl	280 mM	7 mL
1 M MgCl2	20 mM	1 mL
100 mg/mL CHX	0.2 mg/mL	100 μL *Add day of use
1 M DTT	2 mM	100 μL *Add day of use
Milli-Q water	n/a	39.8 mL
Total	n/a	50 mL