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. 2023 Aug 20;13(16):e4757. doi: 10.21769/BioProtoc.4757

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©Copyright : © 2023 The Authors; This is an open access article under the CC BY license

This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).

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Figure 3. — Representative STED images showing cytoskeleton changes associated with differentiation into podocytes based on α-Tubulin (green) and Actin (red) expression in primary human kidney progenitor cells before (A) and after 48 h differentiation (B–G). Compared to confocal microscopy (D, F), the use of STED microscopy and deconvolution software allows to identify the cytoskeleton organization with nanoscopic spatial resolution (C, E, and G).