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| Primary antibody | Secondary antibody | ||||
|---|---|---|---|---|---|
| Antibody | Final concentration | Antibody | Final concentration | Permeabilization | Blocking serum required |
| α-Tubulin | 2 μg/mL | Goat anti-mouse IgG1-Alexa Fluor 594 | 20 μg/mL | Required | Goat |
| Sir-Actin | 1 μM | / | / | Required | / |
| Nephrin (NPHS1) | 4 μg/mL | Donkey anti-sheep IgG(H+L)-Alexa Fluor 488 | 2 μg/mL | Not required | Donkey |
| Podocin (NPHS2) | 30 μg/mL | Goat anti-rabbit IgG(H+L)-Alexa Fluor 546 | 2 μg/mL | Required | Goat |
