| Component | Volume for reaction |
|---|---|
| 10× buffer | 5 μL |
| 25 mM MgCl2 | 3.5 μL |
| dNTPs | 10 μL |
| RNase inhibitor | 2.5 μL |
| Random hexamers | 2.5 μL |
| Multiscribe (MULV) | 2.5 μL |
| RNA | 15 μL |
| RNase-free water | To 50 μL |
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