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(A, D, G) Z. cylindricum; (B, E, H) Z. circumcarinatum; and (C, F, I) Z. Saalach. (A, B, C) Living cells without staining; (D, E, F) acetocarmine staining; (G, H, I) haematoxylin staining. Insets in E, G, and I show manual drawings of chromosomes counted with ImageJ; counted chromosome numbers: n = 20 for Z. cylindricum/Z. circumcarinatum and n = 30 for Z. Saalach. Scale bars = 20 µm.
