Figure 3. Retina tissue collection.

1. Put the mouse in prone position and hold the eyeball from beneath to cause the eye to protrude. 2. Make a wide cut in the cornea along the equator using a sharp blade while holding the eyeball with the forceps from beneath. 3. Grip the dissected eyeball with forceps to remove the lens and vitreous denoted by arrow. 4. Squeeze the remaining eye cup to extract the retina from the eyeball. 5. Move the retina (off-white translucent tissue) to 2–3 mL of cold HBSS. Asterisk denotes the discarded lens with the vitreous and iris. 6. Remove any remaining retinal pigment epithelium (black tissue, denoted by asterisk). 7. Retinas from both eyes in HBSS are ready for further processing.