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. 2023 Jul 17;9(8):1679–1691. doi: 10.1021/acscentsci.3c00611

Figure 8.

Figure 8

Confocal laser scanning microscopy (CLSM) imaging of cell uptake and damage. (a) Subcellular colocalization images of QTCy7-Me/QTCy7-Ph and MitoTracker Green (MTG) in HepG2 cells; P is the colocalization coefficient. (MTG: λex = 488 nm, λem = 500–550 nm; QTCy7-Me/QTCy7-Ph: λex = 640 nm, λem = 700–800 nm; scale bars: 20 μm). (b) Mitochondrial membrane potential detection assays implemented by JC-1 staining. (JC-1 monomer: λex = 488 nm, λem = 500–550 nm; JC-1 aggregate: λex = 488 nm, λem = 560–590 nm; scale bars: 20 μm). (c) QTCy7-Ac and (d) QTCy7-CHO caused cell membrane destruction images at 0–4 min (λex = 640 nm; λem = 650–750 nm; scale bars: 20 μm).