Skip to main content
. 2023 Jul 17;9(8):1679–1691. doi: 10.1021/acscentsci.3c00611

Figure 9.

Figure 9

Intracellular ROS production and cell destruction assays. (a) Images showing intracellular ROS production induced by QTCy7-R under irradiation and dark conditions (λex = 488 nm; λem = 500–550 nm; scale bars: 20 μm) and (b) corresponding fluorescence intensities. (c) Cell viability detection images measured by Calcein-AM/PI for QTCy7-Ac (Calcein-AM: λex = 488 nm, λem = 500–550 nm; PI: λex = 561 nm, λem = 580–630 nm; scale bars: 120 μm). (d) Quantitative detection of cell viability (MTT assay) for QTCy7-R and Ce6 with/without 660 nm irradiation (12 J/cm2). (e) Cell viability of HepG2 cells treated with QTCy7-R at different concentrations under dark condition. (f) Cell viability of HepG2 cells treated with QTCy7-Ac under different light doses. (g) Cell viability of different cancer cells (HepG2, MCF7, and 4T1) treated with QTCy7-Ac under 660 nm irradiation (12 J/cm2). Data are expressed as mean ± SD. **, P < 0.01; ***, p < 0.001; ****, P < 0.0001 as determined by Student’s t test.