Figure 1.

Evaluation of renal damage markers: (A) Creatinine and (B) blood urea nitrogen (BUN) evaluated in plasma; the analysis was conducted on day 7 of evolution (48 h after the last FA administration) using commercial kits. The results are expressed in milligrams per deciliter (mg/dL). Evaluation of liver damage markers: (C) aspartate aminotransferase (AST) and (D) alanine aminotransferase (ALT) in plasma; the analysis was conducted on day 7 of evolution using commercial kits, and the results are expressed in units of the corresponding assays per liter (U/L). Evaluation in plasma of proinflammatory cytokines by Western blot and its densitometry: (E) interleukin one beta (IL-1β) and (F) interleukin six (IL-6). Ponceau S Staining of the corresponding membranes was used as a control charger. Data are mean ± SEM, n = 5–6. ** p < 0.01, *** p < 0.001. FA = Folic Acid, NAC = N-acetyl-cysteine.