Skip to main content
. 2023 Aug 10;12(8):1592. doi: 10.3390/antiox12081592

Figure 9.

Figure 9

Mitochondrial dynamics. Western proteins in the heart isolated mitochondrial and their densitometries of fission protein (A) DRP1 and (B) fusion proteins MFN2. VDAC1 was used as a loading control. Data are mean ± SEM, n = 3. ** p < 0.01. Counting by electron microscopy: (C) total Mitochondrial number and (D) small mitochondria with ovoid or circular morphology per image in cardiomyocyte, as well as (E) fragmentation rate (small/total mitochondria). The electron microscopy images used for counting correspond to a size field of 100 μm. Data are mean ± SEM, n = 7. * p < 0.05, ** p < 0.01, *** p < 0.001. DRP1 immunohistochemistry in heart: (F) Control animal shows scarce DRP1 immunoreactivity. (G) Higher DRP1 immunoreactivity is seen in the cytoplasm of some myocardial cells of the FA group. (H) Rat treated with FA + NAC showed a decrease in DRP1 immunoreactivity in cardiac cells. DRP1 = dynamin-related protein 1, VDAC1 = voltage-dependent anion selective channel 1, MFN2 = mitofusin 2. FA = folic acid, NAC = N-acetyl-cysteine.