Skip to main content
. 2023 Jul 28;12(8):1509. doi: 10.3390/antiox12081509

Figure 3.

Figure 3

Anti-inflammatory activity of OLE, OC and OA in LPS-stimulated HUVEC cells. Cells were pre-treated with OLE (4 μg/mL), OC and OA (5 μM) for 2 h and stimulated with LPS (500 ng/mL) for 3 h. (A) IL-1β, IL-6, TNF-α, IL-8, ICAM-1 and VCAM mRNA expression analysis. Data were reported as fold change vs. untreated LPS-stimulated HUVEC (Ctr) according to 2−ΔΔCt method and using β-actin as housekeeping. (B) IL-6 (pg/mL) in the culture medium; histograms represent the mean of three independent experiments ± SD. Paired t test, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUVEC + LPS (LPS); ## p < 0.01, ### p < 0.001 vs. LPS-untreated HUVEC (Ctr).