Figure 4.

The identified AMPK-dependent phospho-sites in Nrf2 affect the expression of endogenous Nrf2 target genes. (A–D) Keap1-null MEFs were transfected for 48 h with an expression plasmid for HA-tagged β-TrCP2 together with constructs encoding EGFP-MYC-WT-Nrf2 or the triple mutated version EGFP-MYC-TM-Nrf2, as indicated in the absence or presence of the AMPK activator A769662 (50 μM, 4 h and 24 h). RNA was extracted and analyzed for the abundance of Gclc (A,B) and Hmox1 (C,D) by qPCR (TBP as reference gene). Data are presented as means ± SEM. Statistical analysis was performed using multiple comparison one-way ANOVA, with Sidak’s correction, * p < 0.05 and ** p < 0.01. ns: not significant.