TABLE 1.

Oligonucleotides used to generate recombinant fusion proteins of Omp2

Oligonucleotidesa	pEV40-encoded fusion protein	Sizeb (kDA)
Sense: 5′-GAATTCAGCGGGGGTATAGAGGC-3′	C. pneumoniae-Omp2aa23-aa93	10.0
Antisense: 5′-GGATCCTACTGAGCCTCTACAGG-3′
Sense: 5′-GAATTCAGCGGGAAGATAGAGG-3′	C. psittaci-Omp2aa23-aa94	10.9
Antisense: 5′-GGATCCTATGTAGCGTCTACTGG-3′
Sense: 5′-AGCGGGGTGTTAGAGACC-3′	C. trachomatis-Omp2aa23-aa84	9.8
Antisense: 5′-GGATCCTTATTTAGGTCCTGTAGCTTTAG-3′
Sense: 5′-CAGGATTCTTGCTTTGGC-3′	C. trachomatis-Omp2aa87-aa547	53.7
Antisense: 5′-CGGATCCTTAATAGATGTGTGTATTCTCTG-3′
Sense: 5′-AGCGGGGTGTTAGAGACC-3′	C. trachomatis-Omp2aa23-aa182	20.6
Antisense: 5′-CGGGATCCTTAAGGTTTTACCCATACAG-3′
Sense: 5′-CGCTTAGGACAAGGCG-3′	C. trachomatis-Omp2aa167-aa437	32.5
Antisense: 5′-CGGGATCCTTAATGAGTAGCAGCAACTCC-3′
Sense: 5′-TGCGCAGAAGCGACAA-3′	C. trachomatis-Omp2aa420-aa547	17.1
Antisense: 5′-CGGATCCTTAATAGATGTGTGTATTCTCTG-3′

^aUnderlined sequences are of omp2 origin. 

^bTheoretical size of fusion protein.