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. 2023 Aug 17;12(8):1625. doi: 10.3390/antiox12081625

Figure 5.

Figure 5

Histology of hepatic tissue deciphering a comparative effect of sunflower oil (SO) and Raydel ozonated sunflower oil (OSO) on hepatic morphology, fatty liver changes, oxidative stress, and IL-6 production in carboxymethyllysine (CML) injected adult zebrafish at 60 min post-injection. (A) Hematoxylin and eosin (H&E) staining. The black, blue, and red arrows represent the bile duct, arterial area, and portal vein. (B) Oil red O staining. (C) DHE staining for reactive oxygen species detection. (D) IL-6 production [detected by immunocytochemistry (IHC)]. The depicted images are 400× magnified [graphic scale = 0.1 mm], and the stained area was computed employing ImageJ software (version 1.53, http://rsb.info.nih.gov/ij/ retrieved on 16 May 2022). The CML+PBS group was injected with CML (3 mM) dissolved in PBS, whereas CML+(SO1%, SO2%, OSO1%, and OSO2%) groups were co-injected with CML (3 mM) along with either SO1% or SO2% or OSO1% or OSO2%. p value documented the pairwise statistical variation retrieved from the ANOVA employing the Turkey’s test for post hoc analysis.