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. 2023 Aug 17;12(8):1625. doi: 10.3390/antiox12081625

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Multivariate analysis based on tested parameters (survivability, swimming, liver function biomarkers, histology, and blood lipid profile) of zebrafish stimulated by carboxymethyllysine (CML) and subsequently treated with sunflower oil (SO) and Raydel ozonated sunflower oil (OSO). (A,B) are the scoring plot and loading plot, respectively, obtained from principal component analysis (PCA). (C) Hierarchical cluster analysis (HCA). The PCA and HCA were performed using Minitab statistical software version 21.4. The CML+PBS group was injected with CML (3 mM) dissolved in PBS, whereas CML+(SO1%, SO2%, OSO1%, and OSO2%) groups were co-injected with CML (3 mM) and SO1% or SO2% or OSO1% or OSO2%. AST, aspartate aminotransferase; ALT, alanine aminotransferase; HDL-C, high-density lipoprotein cholesterol; TC, total cholesterol; TG, triglyceride.