Figure 2.

MPO reduces apoptosis in A549 cells. A549 cells were treated with MPO (in orange) for 15 h followed by Annexin V and PI staining. (A) Representative flow plots. Gates were set based on single colour staining of AnV and PI. Populations were distinguished as follows: (B) early apoptotic (AnV+/PI−), (C) late apoptotic (AnV+/PI+), (D) alive (AnV−/PI−) and (E) dead (AnV−/PI+) cells. Data indicate mean values ± SD from six independent experiments. Statistical differences were assessed by using one-way ANOVA with Dunnett’s post hoc test * p < 0.05, ** p < 0.01, **** p < 0.0001.