Figure 4.
MPO binds to A549 cells. (A) Cell lysates from MPO-treated A549 cells for 0 (ddH2O = vehicle), 5, 15, 30, 45 and 60 min were analysed via Western blot analysis (N = 4–5). (B) Representative Western blot analysis showing MPO binding to A549 cells after 2 and 24 h of exposure (N = 4). (C) Intracellular flow analysis showing MPO-positive A549 cells as %. Data indicate mean values ± SD from four independent experiments. Statistical differences were assessed using one-way ANOVA with Dunnett´s post hoc test * p < 0.05, ** p< 0.01, *** p< 0.001.