Figure 5.

Transcriptome analysis of the effect of gRc in H₂O₂-treated C2C12 myotubes. (A) Normalized enrichment score (NES) of the gene set enrichment analysis (GSEA) results of H₂O₂ treatment compared to vehicle and the H₂O₂ + gRc treatment compared to the H₂O₂ treatment. The pathway terms were sourced from Gene Ontology—Biological Process (“Regulation of Oxidative Stress Induced Cell Death”, “Positive Regulation of Striated Muscle Cell Differentiation”, “ATP Synthesis Coupled Electron Transport”, “Muscle Cell Proliferation, Cellular Respiration”, and “Positive Regulation of Muscle Hypertrophy”), Gene Ontology—Cellular Component (“Mitochondrial Protein Containing Complex” and “Inner Mitochondrial Membrane Protein Complex”), Reactome (“Apoptosis” and “Myogenesis”), and WikiPathways (“Electron Transport Chain Oxphos System in Mitochondria”). * p < 0.05, ** p < 0.01. (B) The selected GSEA plots of H₂O₂ treatment compared to vehicle (top) and the H₂O₂ + gRc treatment compared to the H₂O₂ treatment (bottom).