Figure 2.

HMP pre-treatment reduced mitochondrial-derived superoxide production in H₂O₂-exposed HTR-8/SVneo trophoblast cells. (a) Representative images from different treatment groups: (A–F): immunofluorescent and (G–L): brightfield pictures per group. Bright red color correlates with superoxide production. Bars: 200 μm. (b) Quantitation of MitoSOX Red immunofluorescence in trophoblasts: Optical density per area (pixel²) of cell surface area was calculated in four high-power fields per sample (n = 5 per group). Mann–Whitney-U-test, median [IQR]. Control vs. 100 μM H₂O₂: **: p < 0.01, 100 μM H₂O₂ vs. H₂O₂ + HMP: **: p < 0.01, 100 μM H₂O₂ vs. H₂O₂ + MitoTEMPO: **: p < 0.01 and H₂O₂ + HMP vs. H₂O₂ + MitoTEMPO: **: p < 0.01.