Figure 3.

HMP pre-treatment reduced HF1A expression in H₂O₂ -exposed HTR-8/SVneo trophoblast cells. (a) Representative images from different treatment groups: (A–F): immunofluorescent and (G–L): brightfield pictures per group. Bright green color correlates with HIF1A expression. Bars: 200 μm. (b) Quantitation of HIF1A immunofluorescence in trophoblasts: Optical density per area (pixel²) of cell surface area was calculated in four high-power fields per sample (n = 5 per group). Mann–Whitney-U test, median [IQR]. Control vs. 100 μM H₂O₂: **: p < 0.01, 100 μM H₂O₂ vs. H₂O₂ + HMP: **: p < 0.01, 100 μM H₂O₂ vs. H₂O₂ + MitoTEMPO: *: p < 0.05 and H₂O₂ + HMP vs. H₂O₂ + MitoTEMPO: **: p < 0.01.