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. 2023 Aug 8;12(8):1578. doi: 10.3390/antiox12081578

Figure 5.

Figure 5

HMP pre-treatment increased the number of active mitochondria in H2O2-exposed HTR-8/SVneo trophoblast cells: (a) Representative images from different treatment groups: (AF): immunofluorescent and (GL): brightfield pictures per group. Bright green color correlates with MitoTracker Green intensity. Bars: 200 μm. (b) Quantitation of MitoTracker Green immunofluorescence in trophoblasts: Optical density per area (pixel2) of cell surface area was calculated in four high-power fields per sample (n = 5 per group). Mann–Whitney-U test, median [IQR]. Control vs. 100 μM H2O2: **: p < 0.01, 100 μM H2O2 vs. H2O2 + HMP: **: p < 0.01, 100 μM H2O2 vs. H2O2 + MitoTEMPO: **: p < 0.01 and H2O2 + HMP vs. H2O2 + MitoTEMPO: **: p < 0.01.