FIG. 1.

Time course of cytokine mRNA production and accumulation after stimulation. PBMC were stimulated with PHA (A) and with anti-CD3 monoclonal antibody (B). Total cellular RNA was isolated at the indicated times after activation. mRNA encoding IL-2 (⧫), IL-2R (▪), IL-6 (▴), IL-10 (×), TNF-α (*), and IFN-γ (•) was measured by the semiquantitative RT-PCR method. Data for samples from four healthy donors are presented as mean proportions of the maximal amounts of each specific mRNA.