TABLE 1.

Cytokine primers used for PCR

Gene product (direction of strand)	Fragment size (bases)	Primer sequence
IL-2 (5′)	222	5′-GAATGGAATTAATAATTACAAGAATCCC-3′
IL-2 (3′)	222	5′-TGTTTCAGACCCTTTAGTTCAG-3′
TNF-α (5′)	325	5′-CAGAGGGAAGAGTTLCCCAG-3′
TNF-α (3′)	325	5′-CCTTGGTCTGGATAGGAGACG-3′
IL-10 (5′)	352	5′-ATGCCCCAAGCTGAGAACCAAGACCCA-3′
IL-10 (3′)	352	5′-TCTCAAGGGGCTGGGTCAGCTATCCCA-3
IFN-γ (5′)	520	5′-ATGAAATATACAAGTTATAGC-3′
IFN-γ (3′)	520	5′-TTACTGGGATGCTCTTCGACCTCGAAACAGCAT-3′
IL-6 (3′)	190	5′-ATGTAGCCGCCCCACACAGA-3′
IL-6 (3′)	190	5′-CATCCATCTTTTTCAGCCAT-3′
IL-2R (5′)	398	5′-GAATTTATCATTTCGTGGTGGGGCA-3′
IL-2R (3′)	398	5′-TCTTCTACTCTTCCTCTGTCTCCG-3′
β-Actin (5′)	1,126	5′-ATGGATGATGATATCGCCGCC-3′
β-Actin (3′)	1,126	5′-CTAGAAGCATTTGCGGTGGACGATGGAGGGGCC-3′