TABLE 2.
Cytokine mRNA levels in unstimulated PBMC
| mRNA | mRNA level in PBMC from subjecta
|
|||
|---|---|---|---|---|
| DC-A
|
DC-B
|
|||
| Immediately after cell separation | After restingb | Immediately after cell separation | After restingb | |
| IL-2 | 14.6 | 5.7 | 0.4 | 0.1 |
| IL-2R | 13.5 | 18.9 | 3.3 | 2.7 |
| IL-6 | 26.0 | 21.8 | 30.0 | 13.2 |
| IL-10 | 4.9 | 3.0 | 2.0 | 0.8 |
| TNF-α | 25.7 | 13.2 | 3.2 | 2.9 |
| IFN-γ | 23.3 | 7.7 | 5.0 | 0.3 |
a
Percentage of the maximum mRNA expression upon stimulation with PHA shown in Fig. 1.
b
Minimum mRNA levels in unstimulated PBMC found within 4 to 72 h of culture.