TABLE 3.
Maximum level of induced cytokine mRNA and time of maximal response
| Stimulus and subject | mRNA ratioa
|
|||||
|---|---|---|---|---|---|---|
| IL-2 | IL-2R | IL-6 | IL-10 | TNF-α | IFN-γ | |
| PHA | ||||||
| DC-1 | 29 (8) | 51 (72) | 15 (24) | 2 (8) | 34 (8) | 78 (8) |
| DC-2 | 3 (8) | 18 (8) | 115 (8) | 7 (8) | 52 (8) | 22 (24) |
| DC-A | 7 (4) | 7 (4) | 4 (4) | 21 (4) | 4 (8) | 4 (4) |
| DC-B | 265 (8) | 30 (8) | 3 (8) | 49 (8) | 31 (8) | 20 (8) |
| Anti-CD3 | ||||||
| DC-1 | 12 (8) | 54 (72) | 5 (8) | 3 (8) | 19 (8) | 91 (8) |
| DC-2 | 3 (4) | 12 (72) | 69 (8) | 15 (8) | 50 (8) | 20 (24) |
| DC-A | 10 (4) | 6 (72) | 3 (8) | 43 (8) | 6 (4) | 6 (4) |
| DC-B | 277 (4) | 13 (72) | 3 (4) | 58 (8) | 11 (8) | 41 (8) |
a
In samples from four healthy donors, the ratio of the maximum amount of mRNA (in counts per minute) upon stimulation with PHA or anti-CD3 to the amount of mRNA (in counts per minute) in unstimulated cells was determined. The time (in hours) after stimulation when the maximum level of mRNA was reached is indicated in parentheses.