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. 2023 Jul 29;12(8):1064. doi: 10.3390/biology12081064

Figure 5.

Figure 5

Immunolabeling of triadic proteins (and EM of longitudinal triads) in WT and CASQ1-null adult (4-month-old) fibers. (A–D) Confocal images of CASQ1-null EDL fibers immunolabeled with antibodies against RyR, DHPR, and triadin (TRN). (E–H) Higher magnification confocal images: arrows in (G,H) point to immuno-positive RyR and DHPR foci longitudinally oriented, which are not present on WT. (I) EM of longitudinally oriented triads in a CASQ1-null fiber. Bars: (A–D), 5 µm; (E–F), 1 mm; (I), 0.1 µm.