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. 2023 Jul 26;13(8):1168. doi: 10.3390/biom13081168

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Extracted compound chromatograms (ECCs) of cucurbitacin G/H (orange, 1 and 2), J/K (yellow, 3 and 4), D (purple, 5), I (red, 7), B (green, 9), and E (blue, 11) and of 23,24-dihydrocucurbitacin D (light purple, 6), I (light red, 8), B (light green, 10), and E (light blue, 12) of the HPLC-QTOF-MS analysis of the ethyl acetate extract of the tuber of C. naudinianus (see Table 2). HPLC settings: ZORBAX Eclipse Plus C18 RRHD Column (50 × 3 mm; 1.8 µm); solvent gradient of water + 0.1% FA (A) and acetonitrile:isopropanol (9:1) + 0.1% FA (B); column temperature, 40 °C; flow rate 0.6 mL/min. MS settings: Negative mode; capillary voltage, 3000 V; fragmentor voltage, 135 V; skimmer voltage, 65 V.