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Bioprinted heart valves. (i) Extrusion-based bioprinted heart valve containing alginate/gelatin encapsulated with VIC/SMC, respectively, with (ii) fluorescent cell labelling of SMCs in green and VICs in red and the expression of aSMA and vimentin after 7 day culture and inset live/dead assay for encapsulated (iii) VIC and (iv) SMC. ** p < 0.01. Reprinted with permission from Duan et al. [142].
