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. 2023 Jul 28;13(8):1180. doi: 10.3390/biom13081180

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Simplified extrusion bioprinted heart valve printed from Me-Gel encapsulated with HAVIC with (i) live/dead staining showing cell viability through a cross section of the valve from surface to 300 um, (ii) inset showing simplified morphology, (iii–v) histological staining of bioprinted leaflets after 7 day culture. (iii) Safranin-O staining was used to stain the GAGs red, which also stained the Me-HA within the hydrogel red, (iv) Masson’s Trichrome staining was used to stain collagen blue. Reprinted with permission from Duan et al. [143].