TABLE 1.
Serotype, serosubtype, and PorB VR type characteristics of N. meningitidis strains analyzed
| Strain | Sourceh | Serogroup | Serotype
|
Serosubtypef | Accession no.c | PorB protein classd | PorB VR typee
|
||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Originala | Proposedb | 1 | 2 | 3 | 4 | ||||||
| Prototype strains | |||||||||||
| M1080 | FDA | B | 1 | 19,7,1 | P1.7,1 | X65530 | 3 | 19 | Ac | 7a | 1 |
| B16B6 | FDA | B | 2a | 2a | P1.5,2 | X67937 | 2 | C | Eb | 2a | C |
| 2996 | FDA | B | 2b | 2b | P1.5,2 | X67939 | 2 | C | Ea | 2b | C |
| 2396 | SIF | B | 2c | 2c | P1.5,2 | U92911 | 2 | C | 2c | 2bb | Db |
| 126E | SIF | C | 3 | 19,10 | P1.5,2 | U07191 | 3 | 19 | Ac | 10 | 14a |
| Z-4008j | MPIG | B | 4 | 4,7 | P1.4 | U59868 | 3 | 4 | D | 7 | 14a |
| H-276k | MPIG | B | 4 | 4,7 | P1.10 | U59870 | 3 | 4 | D | 7 | 14a |
| M981 | FDA | B | 4 | 4,7 | P1− | X65531 | 3 | 4 | B | 7 | 14a |
| M992 | FDA | B | 5 | 5 | P1.7,1 | U92909 | 2 | Ca | Ec | 5 | Ca |
| M990 | FDA | B | 6 | 16,6 | P1.6 | X67936 | 2 | D | 16 | 6 | D |
| M978 | FDA | B | 8 | 19,10 | P1.7,1 | U07189 | 3 | 19 | Aa | 10 | 14a |
| M982 | FDA | B | 9 | NT | P1.9 | X67938 | 2 | C | E | B | Da |
| N.34/94 | IAL | B | NTi | 19,10 | P1.4 | U34194 | 3 | 19 | Db | 10 | Aa |
| M136 | SIF | B | 11 | 16,11 | P1− | U95366 | 2 | D | 16 | 11 | D |
| S3032 | SIF | B | 12 | 19,7 | P1.12,16 | X65534 | 3 | 19 | Ab | 7a | A |
| S3446 | SIF | B | 14 | 19,14 | P1.23,14 | U07188 | 3 | 19 | Db | 7c | 14 |
| H.44/76 | SIF | B | 15 | 15 | P1.7,16 | X83428 | 3 | A | A | A | Ba |
| BB393 | FDA | B | 15 | 15 | P1.3 | U62905 | 3 | A | A | A | Ba |
| H355 | FDA | B | 15 | 15 | P1.15 | M68962 | 3 | A | A | A | B |
| 60E | FDA | C | 16 | 16,2bg | P1.7,1 | U92908 | 2 | C | 16 | 2ba | Db |
| 6557 | FDA | B | 17 | 17,7 | P1.23,14 | U07190 | 3 | B | C | 7 | 14b |
| 190I | FDA | B | 18 | 19,10 | P1.6 | U07192 | 3 | 19 | Aa | 10 | 14a |
| 6940 | FDA | B | 19 | 19,10 | P1.6 | U11030 | 3 | 19 | Ac | 10 | 14a |
| 35E | SIF | C | 20 | 2c | P1.1 | X67940 | 2 | C | 2c | 2bb | Db |
| M1027 | FDA | A | 21 | 4,21 | P1− | U59869 | 3 | (4) | D | 7b | (21) |
| 503/93 | NIPH | B | 22 | NT | nt | U92906 | 2 | C | Ed | 5a | Db |
| Additional strains | |||||||||||
| 94010 | LCDCTP | C | 2a | 2a | P1.5,2 | U92907 | 2 | C | Eb | 2a | C |
| M986 | FDA | B | 2a | 2a | P1.5,2 | U92912 | 2 | C | Eb | 2a | C |
| N.446/95 | IAL | C | 2b | 2b | P1.10 | U92910 | 2 | C | Ea | 2b | C |
| N.781/95 | IAL | C | 2b | 2b | P1.10 | U92913 | 2 | C | Ea | 2b | C |
| N.862/95 | IAL | C | 2b | 2b | nt | U94962 | 2 | C | Ea | 2b | C |
| N.1302/95 | IAL | B | 2b | 2b | P1.5 | U92905 | 2 | C | Ea | 2b | C |
| N.459/93 | IAL | B | 4 | 4,7 | P1.7,1 | U92903 | 3 | 4 | Da | 7 | 14a |
| N.585/94 | IAL | B | 4 | 4,7 | P1.13 | U92899 | 3 | 4 | B | 7 | 14a |
| N.300/94 | IAL | B | 4 | 4,7 | P1.9 | U59866 | 3 | 4 | B | 7 | 14a |
| CU385 | FDA | B | 4 | 4,7 | P1.15 | L03786 | 3 | 4 | B | 7 | 14a |
| N.1281/95 | IAL | B | 4 | 4,7 | nt | AF012017 | 3 | 4 | B | 7 | 14a |
| N.150/88 | IAL | B | 4 | 4,7 | P1.15 | U59872 | 3 | 4 | D | 7 | 14a |
| N.44/89 | IAL | B | 4 | 4,7 | P1.15 | U59871 | 3 | 4 | D | 7 | 14a |
| N.155/94 | IAL | B | 4 | 4,7 | P1.15 | U94960 | 3 | 4 | D | 7 | 14a |
| N.610/93 | IAL | B | 4 | 4,7 | P1.15 | U59867 | 3 | 4 | D | 7 | 14a |
| N.314/95 | IAL | B | 4 | 4,7 | P1.15 | AF012015 | 3 | 4 | D | 7 | 14a |
| N.454/95 | IAL | B | 4 | 4,7 | P1.15 | AF012013 | 3 | 4 | D | 7 | 14a |
| N.45/95 | IAL | B | 4 | 4,7 | P1.15 | AF012014 | 3 | 4 | D | 7 | 14a |
| 501 | CDC | B | 4,15 | 4,15 | nt | AF002250 | 3 | 4 | A | A | Ba |
| N.294/97 | IAL | B | 15 | 15 | P1.16 | AF012018 | 3 | A | A | A | Ba |
| N.109/93 | IAL | B | 4 | 4,10 | P1.9 | U92904 | 3 | 4b | D | 10 | 14a |
| N.23/97 | IAL | B | 4 | 4,10 | P1.15 | AF001321 | 3 | 4 | D | 10 | 14a |
| N.520/95 | IAL | B | 4 | 4,10 | P1.15 | AF012016 | 3 | 4 | B | 10 | 14c |
| N.163/94 | IAL | B | 8 | 19,10 | P1.6 | U92902 | 3 | 19 | Aa | 10 | 14a |
| N.19/93 | IAL | B | 7 | 7 | nt | U92901 | 3 | A | D | 7d | 14a |
| N.105/93 | IAL | B | 17 | 17,7 | nt | U92900 | 3 | B | C | 7 | 14b |
| N.1342/96 | IAL | B | 17 | 17,7 | P1.7,16 | AF001322 | 3 | B | C | 7 | 14b |
| N.1329/96 | IAL | B | 17 | 17,7 | P1.7,16 | AF001319 | 3 | B | C | 7 | 14b |
| N.405/94 | IAL | B | 19 | 19,7,1 | nt | U94961 | 3 | 19 | Ac | 7a | 1 |
| N.1434/96 | IAL | B | 19 | 19,14 | P1.23,3 | AF001323 | 3 | 19 | Db | 7c | 14 |
| N.1450/96 | IAL | B | 19 | 19,14 | P1.23,3 | AF001320 | 3 | 19 | Db | 7c | 14 |
| N.1454/96 | IAL | B | 19 | 19,14 | P1.23,3 | AF001318 | 3 | 19 | Db | 7c | 14 |
| N.257/93 | IAL | B | NT | NT | nt | U92914 | 2 | Cb | Ed | C | Ca |
| J129 | GB | B | 4 | (4),(7)h | P1.15 | X67933 | 3 | 4a | D | 7 | 14a |
| BB1350 | GB | B | 4 | (4),(7) | UK | U07193 | 3 | 4 | D | 7 | 14a |
| PM36 | GB | B | 4 | (4),(7) | P1.15 | X79464 | 3 | 4 | B | 7 | Bb |
| PM40 | GB | B | 4 | (4),(7) | P1.2 | X78579 | 3 | 4 | B | 7 | 14a |
| B54 | GB | A | 4,21 | (4),(21) | P1.X,9 | X67934 | 3 | 4 | D | 7b | 21 |
| B227 | GB | A | 4,21 | (4),(21) | P1.5,9 | X67935 | 3 | 4 | D | 7b | 21 |
| G1960 | GB | B | 15 | (15) | P1.7,16 | X67932 | 3 | Aa | A | A | Ba |
| MC58 | GB | B | 15 | (15) | UK | X65532 | 3 | A | A | A | Ba |
Original serotypes were defined by Frasch et al. (9) by using rabbit immunosera and MAbs.
Proposed serotypes were characterized by dot blotting reactions using serotype-defining MAbs, including the new MAbs 7, 10, and 19 as described in the text. Because we did not have access to eight meningococcal strains from sequences obtained from the GenBank, the serotyping of those strains was not repeated in our laboratory; the serotypes based on predicted reactions with the serotype-defining MAbs are presented in parentheses.
Boldface GenBank accession numbers are those for which the nucleotide sequences were determined in this work.
PorB protein classes were defined by SDS-PAGE (24).
PorB VR types were defined by a comparison of MAb reactivities and amino acid sequence homology as described in the text.
P1−, no expression of PorA class 1 protein by SDS-PAGE; nt, nonserosubtypeable; UK, unknown serosubtype.
VR3-2ba can only be detected by dot blotting using serotype-defining MAb 2b (F1-9H10/1B3) or 1082E7.
MPIG, Max-Planck-Institut Fur Molekulare Genetic; LCDCTP, Laboratory Center for Disease Control, Tunney’s Pasture. GB, GenBank.
NT, nonserotypeable.
882066.
870227.