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. 1998 May;5(3):348–354. doi: 10.1128/cdli.5.3.348-354.1998

TABLE 3.

Characteristics of porB gene VR nucleotide sequences, PorB protein VR families, and types of N. meningitidis

Loop PorB VR typea PorB protein classb Reactivity with serotype-defining MAbc Strain Sequenced
Accession no.
VR nucleotides VR amino acids
124                           156 42       52
I VR1-4 3 + M981 GAGCACAATGGAGGTCAGGTGGTTAGCGTTGAA EHNGGQVVSVE X65531
VR1-[4] 3 + M1027 ..A.............................. ........... U59869
VR1-4a 3 (+) J129 ........G........................ ..K........ X67933
VR1-4b 3 + N.109/93 A................................ K.......... U92904
VR1-19 3 + 6940 GCTCACAATGGAGCTCAGGCGGCTAGCGTTGAA AHNGAQAASVE U11030
VR1-A 3 NAA H355 TTTCACCAGAACGGCCAAGTTACTGAAGTTACA FHQNGQVTEVT M68962
VR1-Aa 3 NAA G1960 ..............................G.. ..........A X67932
VR1-B 3 NAA 6557 TATTACGAAGACGGCAAAGCTGCTGAAGTTACA YYEDGKAAEVT U07190
19                               54 7        18
VR1-C 2 NAA B16B6 GATGCTGGTACATATAAAGCTCAAGGCGGAAAATCT DAGTYKAQGGKS X67937
VR1-Ca 2 NAA M992 .......C...........A................ ..A...D..... U92909
VR1-Cb 2 NAA N.257/93 .......A............................ ..D......... U92914
VR1-D 2 NAA M990 AATCAAGGCGTAAAAACC NQGVKT X67936
610                  633 204  211
V VR2-A 3 NAA H355 CATCAAGTGCAAGAGGGCTTGAAT HQVQEGLN M68962
VR2-Aa 3 NAA M978 ........A.......A....... .....D.. U07189
VR2-Ab 3 NAA S3032 ....G...........A.A.A... .R...DI. X65534
VR2-Ac 3 NAA M1080 ...............AA.G..... .....NV. X65530
VR2-B 3 NAA M981 GTGCGGGTGGATGAGAACGTGAAT VRVDENVN X65531
VR2-C 3 NAA 6557 GAGCAGATAGATAACGTGAAG EQIDNVK U07190
VR2-D 3 NAA J129 CAGGATGTGGATGACGTGAAG QDVDDVK X67933
VR2-Da 3 NAA N.459/93 .........A........... ...N... U92903
VR2-Db 3 NAA N.34/94 ...A........A........ .N..N.. U34194
556                                          603 186          201
VR2-2c 2 + 2396 AACACTGATGCAGAACGTGTTGCAGTAGGTGCTACAGATGCCAATCCT NTDAERVAVGATDANP U92911
VR2-16 2 + 60E AACAAGGATGCAGAACGTGTTGCAGCAGGTACTTCAGGTGCCTATGCT NKDAERVAAGTSGAYA U92908
VR2-E 2 NAA M982 AACAATGATGCAGAACGTGTTGCAGTAAATACTCCAAATGCCCATGCT NNDAERVAVNTPNAHA X67938
VR2-Ea 2 NAA 2996 ..AG..A..........................G...........C.. KDN........A...P X67939
VR2-Eb 2 NAA B16B6 ....C............................G...........C.. .T.........A...P X67937
VR2-Ec 2 NAA M992 ...G.............................A.............. .D.........T.... U92909
VR2-Ed 2 NAA N.257/93 ..AG.TA.......G............GG....A..GG.......C.. KDN......G.TG..P U92914
727         741 243 247
VI VR3-7 3 + H-276 GTTGAAGACAATTAT VEDNY U59870
VR3-7a 3 + S3032 ........A...... ..E.. X65534
VR3-7b 3 − B54 ............––– ....– X67934
VR3-7c 3 − S3446 ...A........––– .K..– U07188
VR3-7d 3 + N.19/93 ...A........... .K... U92901
VR3-10 3 + N.34/94 GCTTTGCCAAACGACAAT ALPNDN U34194
VR3-A 3 NAA H355 ACTGATGCTTCCAAT TDASN M68962
694                  717 232   239
VR3-2a 2 + B16B6 AACAACGAGGTTGGTTCTACCAAG NNEVGSTK X67937
VR3-2b 2 + 2996 AATAACACTGGTACTGTCGGCAAACAA NNTGTVGKQ X67939
VR3-2ba 2 −/+ 60E .....................G..... .......E. U92908
VR3-2bb 2 − 35E ..........T..G.......G..... ...VS..E. X67940
VR3-5 2 + M992 AACAACGACGGTTCTGCCAATAAT NNDGSANN U92909
VR3-5a 2 − 503/93 ........T...A........C.A ....T..Q U92906
VR3-6 2 + M990 CAA Q X67936
VR3-11 2 + M136 AACAACGAAGGTGCTGCTAAACAA NNEGAAKQ U95366
VR3-B 2 NAA M982 AACAACGAGGTTGGTAATCTCAATATT NNEVGNLNI X67938
VR3-C 2 NAA N.257/93 AATAACACCGGTGTTGCCGCCGATCAA NNTGVAADQ U92914
835                        864 279    288
VII VR4-1 3 + M1080 TCGTTTGATGCTACAAACTACAACAACGAT SFDATNYNND X65530
VR4-14 3 + S3446 TCGTTTGATGATGCAGACTACACCAACGAT SFDDADYTND U07188
VR4-14a 3 − M978 ...................TA.G....... ......LS.. U07189
VR4-14b 3 − 6557 ..................CG.TA...TAC. ......RY.T U07190
VR4-14c 3 − N.520/95 .........A.........TA.G....... ...N..LS.. AF01201
VR4-21 3 + M1027 TCGGTTGATGATGCAAAACGCGACAATACT SVDDAKRDNT U59869
VR4-[21] 3 + B54 ..A........................... .......... X67934
VR4-Ae 3 NAA S3032 TTGGTTGATAGTGCAGACTACACCAACGAT LVDSADYTND X65534
VR4-Aa 3 NAA N.34/94 ...................TA.G....... ......LS.. U34194
VR4-B 3 NAA H355 TTGGTTGATAATGCAGACATAGGCAACGAA LVDNADIGNE M68962
VR4-Ba 3 NAA MC58 .........G.................... ...D...... X65532
VR4-Bb 3 NAA PM36 .C.T.......................... SF........ X79464
826         840 277 281
VR4-C 2 NAA B16B6 GTGAAAGACGCAAAT VKDAN X67937
VR4-Ca 2 NAA M992 .............G. ....S U92909
VR4-D 2 NAA M990 GAGAAAGTAGCACGT EKVAR X67936
VR4-Da 2 NAA M982 .......C....... ..A.. X67938
VR4-Db 2 NAA 35E A......C....... K.A.. X67940
a

PorB VR types were defined by comparison of MAb reactivities and amino acid sequence similarity as described in the text. Brackets indicate that nucleotide sequence differences produce no amino acid changes with respect to the prototype. 

b

PorB protein classes were defined by SDS-PAGE (24). 

c

Reactivity with serotype-defining MAbs was evaluated by dot blotting and immunoblotting reactions; the results were 100% concordant. Immunoblot reactions were positive only in the presence of detergent Empigen BB. +, positive reaction; −, negative reaction; −/+, the reaction can be negative or positive depending on the MAb used; see text. Parentheses indicate that the reactivity is not based in experimental evidence but rather is predicted. NAA, no antibody available. 

d

The numbers above the sequences refer to the nucleotide or amino acid VR position, based on the porB gene sequence of B16B6 strain for the class 2 protein and S3032 strain for the class 3 protein; conserved regions of the loops were not considered part of the VRs. –, nucleotide or amino acid deletion; ., nucleotide or amino acid identical to that of the prototype. 

e

For serotype 12, the corresponding epitope is most probably VR4-A(2), but since there is no MAb 12 available, we did not consider this designation.