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. 2023 Aug 11;15(16):4063. doi: 10.3390/cancers15164063

Figure 7.

Figure 7

Inhibitory effect of CR, SB, and CR + SB on HCC-induced upregulation in NF-kB-p65 and COX-2 expression in liver. (A) Representative images of IHC NF-kB-p65 and COX-2 stained sections in the livers of all groups studied. (B,C) represent the quantification of COX-2 and NF-kB-p65-positive staining area. Increased NF-kB-p65 and COX-2 immunopositivity staining was observed in livers of the HCC group, as indicated by a brown color, as compared to the control group. The percent of positive staining area was reduced in SB, CR, and CR + SB treated groups. The expression of NF-kB-p65 and COX-2 in each section was determined by calculating the percent of positive area of brown staining in ten fields at ×400 magnifications and then the area of positive cells/field. Values expressed as mean ± SEM of six animals in each group. Significance was determined by one-way analysis of variance followed by Tukey’s post hoc analysis: a p < 0.05 vs. control group; b p < 0.05 vs. HCC group; c p < 0.05 vs. HCC + SB group, d p < 0.05 vs. HCC + CR group.