Table 1.

The HLA class I polyreactivity of commercial anti-HLA-E mAbs (MEM-E/02, MEM-E/06, MEM-E/07, MEM-E/08, 3D12), claimed to be HLA-E-specific, is compared with truly polyreactive mAb TFL-006 [47,48,49] in contrast to the HLA-I non-reactive, HLA-E monospecific mAb TFL-033 [40,41,42,43,44]. The HLA reactivity of the mAbs was examined with the single antigen bead assay using Luminex xMAP multiplex technology with dual-laser flow cytometry. The data are based on the LABScreen xMAP microbeads coated with an admixture of B2m-free and B2m-associated array of HLA antigens representing various alleles [45,46]. TFL-006 was also tested with LIFECODES xMAP microbeads coated only with intact B2m-associated HLA alleles [49] and all antigen-coated beads gave negative results, indicating that TFL-006 does not bind to Face-1.

HLA-Ia Alleles	mAb	mAb	mAb	mAb MEM Series				HLA-Ia Alleles	mAb	mAb	mAb	mAb MEM Series
	TFL-033	TFL-006	3D12	E/02	E/06	E/07	E/08		TFL-033	TFL-006	3D12	E/02	E/06	E/07	E/08
	HLA-E specific	HLA-E non-specific mAbs							HLA-E specific	HLA-E non-specific mAbs
A*01:01		1684			11,453			B*07:02		1080		1910	12,951	1375	618
A*03:01		649	789		9525			B*08:01		2422		1062	15,204		4281
A*11:01		4600		2940	7067	4992	1321	B*13:01		1956	1820	5700	15,934	6182	3195
A*11:02		607		559	9404	580		B*13:02		5604	1171	1326	16,249	393
A*23:01					12,666			B*14:01		2919		3135	14,876	4103	1308
A*24:02		1256		4096	14,316	10,991	2739	B*14:02				942	12,447		634
A*24:03		1924		2505	15,894	9981	2094	B*15:01		3440		832	14,606
A*25:01		788		629				B*15:02		1345		3250	15,676	3860	1051
A*29:02		1204		1593		1770	643	B*15:03		1874		4731	15,401		571
A*30:01		2232		526	10,072		551	B*15:10		1292		768	15,482
A*30:02		1368			5296			B*15:12		2619		1903	15,670	850
A*32:01		955		603				B*15:13		2833	591	3400	15,450	4023	982
A*33:01		1079		3037	1195	3469	1163	B*15:16		3503			13,159
A*33:03		1204		1604		1228	511	B*18:01		1352		4392	16,138	3923	1665
A*34:01		2362		991	757	754		B*27:05		3518		942	13,985
A*36:01		4993		1219	9451	1493		B*27:08		4668	3264	1175	14,289	640
A*66:01		1783		571				B*35:01		2834	566	8716	15,768	12,917	6233
A*68:01		4150		664				B*37:01		1137		3444	14,356	3109	1871
A*69:01		992		917				B*38:01		6219	1672	968	14,774	516
C*01:02		3238	966	3125	12,748	2998	3217	B*39:01		3301		3010	12,219	3825	1289
C*02:02		5570	720	2567	17,083	2003	1986	B*40:01		8083	800	3478	15,269	2662
C*03:02		15,443		1713	14,941	1705	624	B*40:02		763	712	2442	9971	2166	631
C*0303		5990	571	2358	14,248	1549	1067	B*40:06		4678	3216	9898	15,642	14,269	6208
C*03:04		10,870		2585	13,686	1875	903	B*41:01		3037		4987	14,635	5400	1331
C*04:03		5809	3796	1765	7077	2052	703	B*42:01		2163			14,286
C*05:01		3635	931	9263	15,923	15,435	6346	B*44:02		1537		2621	15,821	1525
C*06:02		8171		3076	17,836	1914	10,260	B*44:03		1584	1321	2654	13,339	1625
C*07:02		3082	1640	6680	1911	7149	13,655	B*45:01		4122	604	3134	14,671	3234	748
C*08:01		8093	592	2481	15,461	2001		B*46:01		4176		3042	16,173	3389	1062
C*12:03		9256	1020	1692	15,372	1035	771	B*47:01		2630		777	8849
C*14:02		5143		1889	12,570	1839	1637	B*48:01		2822		3577	10,982		514
C*15:02		5101		2688	16,008	918	1080	B*49:01		3201		1588	15,804
C*16:01		3833	530	1128	15,803	590	735	B*50:01		4895		769	15,575
C*17:01		7054	5554	1869		1361	841	B*51:01		6464	841	2485	13,576	2619	884
C*18:02		1402	1095	7779	14,530	11,223	8373	B*51:02		3766		2303	13,138	2352	800
								B*52:01		1168	1416	928	8975
								B*53:01		2324		2754	13,622	2890	1384
								B*54:01		1186		1910	13,506	1274	598
								B*55:01		3525		1287	14,420	892	180
								B*56:01		6569		5352	16,067	5075	1881
								B*57:01		4535	588	3626	11,746	5398	1982
								B*57:03		388	1143	2586	14,723	3272	1556
								B*58:01		2668	823	1636	11,721	1809	1155
								B*59:01		4289		2803	16,222	1837	915
								B*67:01		2308	1856	704	12,533
								B*73:01		5661	659	5560	2363	8347	3629
								B*78:01		6082		4273	11,454	5927	1678
								B*81:01		7621	579	1097	11,758
								B*82:01		4317		5295	15,480	6315	1922