Figure 3.

The mRNA expression levels of integrins in pre-osteoblasts and fibroblasts. (a) The mRNA expression level of integrin subunits that specifically bind type I collagen and fibronectin in pre-osteoblasts and fibroblasts. Data are presented as mean ± standard error of the mean, * p < 0.05, ** p < 0.01, *** p < 0.001. ns refers to no statistically significant difference. (b) Differences in integrin subunit mRNA expression levels in fibroblasts compared to pre-osteoblasts. Itga10 and Itga11 (blue) are at a lower expression level in fibroblasts while Itgb1, Itgb3, and ItgaV (red) are at a higher expression level in fibroblasts compared to pre-osteoblast. There was no statistically significant difference of Itga1, Itga2 and Itga5 (grey) expression level in pre-osteoblasts and fibroblasts. Three independent experiments (n = 3).