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Composition of Acrylamide/Bisacrylamide for Preparation of Polyacrylamide Substrate.
| Soft | Middle | Stiff | |
|---|---|---|---|
| 40% Acrylamide (mL) | 1.25 | 1.88 | 3.00 |
| 2% Bisacrylamide (mL) | 0.75 | 1.50 | 3.00 |
| 10% Ammonium persulfate (mL) | 0.1 | 0.1 | 0.1 |
| TEMED (mL) | 0.1 | 0.1 | 0.1 |
| ddH2O (mL) | 7.8 | 6.42 | 3.8 |
