Table 3.
Average Ct values (Ctgene − Ctactin) for each S100 and for HMGB1 gene, in each of the 8 cancer cell lines and in the HPNE non-cancerous control cell line tested. The standard deviations are indicated below each mean value. The mean Ct and its corresponding standard deviation for each ligand across all 8 pancreatic cancer cell lines cell lines is also indicated. ND: not detectable.
| Cell Line | A2 | A4 | A6 | A7 | A8 | A9 | A10 | A11 | A12 | A13 | A14 | A16 | P | HMGB1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AsPC-1 | 17.35 +/− 3.53 |
1.86 +/− 0.26 |
11.04 +/− 1.75 |
15.9 +/− 0.53 |
13.27 +/− 0.99 |
12.25 +/− 1.00 |
3.34 +/− 0.90 |
7.07 +/− 1.37 | 9.26 +/− 0.21 | 8.33 +/− 0.11 | 11.33 +/− 0.72 |
6.25 +/− 1.02 |
8.89 +/− 0.61 |
7.33 +/− 0.62 |
| BxPC-3 | 9.17 +/− 1.78 |
6.99 +/− 1.18 |
12.89 +/− 0.81 |
13.17 +/− 0.30 |
10.95 +/− 0.50 |
7.17 +/− 1.01 |
4.28 +/− 0.68 |
5.38 +/− 1.29 |
8.50 +/− 0.17 |
5.96 +/− 0.01 |
8.95 +/− 1.14 |
7.63 +/− 0.44 |
4.18 +/− 0.18 |
7.25 +/− 0.18 |
| Capan-2 | 17.1 +/− 0.79 |
5.97 +/− 0.32 |
11.82 +/− 0.42 |
14.45 +/− 0.26 |
13.05 +/− 0.61 |
12.08 +/− 0.41 |
3.68 +/− 0.57 |
6.47 +/− 0.09 |
9.51 +/− 0.08 |
6.52 +/− 0.01 |
11.07 +/− 0.88 |
8.37 +/− 0.16 |
7.65 +/− 0.25 |
7.10 +/− 0.77 |
| CFPAC-1 | 17.88 +/− 1.06 |
10.13 +/− 0.73 |
13.09 +/− 0.54 |
12.12 +/− 0.21 |
12.79 +/− 1.37 |
9.84 +/− 0.13 |
4.79 +/− 0.62 |
7.22 +/− 0.25 |
6.54 +/− 0.2 |
7.31 +/− 0.03 |
12.28 +/− 1.02 | 7.80 +/− 0.48 |
10.8 +/− 0.17 |
8.67 +/− 0.32 |
| HPAF-2 | 17.59 +/− 0.77 |
7.84 +/− 0.45 |
12.25 +/− 0.43 |
15.16 +/− 0.22 |
10.85 +/− 1.63 |
10.42 +/− 1.14 |
3.36 +/− 0.19 |
6.59 +/− 0.55 |
10.38 +/− 0.13 |
6.71 +/− 0.08 |
10.29 +/− 1.36 |
9.16 +/− 0.20 |
3.88 +/− 0.63 |
7.33 +/− 1.23 |
| Hs766T | 11.4 +/− 0.65 |
1.91 +/− 0.51 |
11.67 +/− 0.38 |
12.70 +/− 0.29 |
12.70 +/− 2.28 |
11.03 +/− 0.19 |
3.72 +/− 0.52 |
7.00 +/− 0.56 |
7.95 +/− 0.07 |
5.27 +/− 0.01 |
19.32 +/− 0.82 |
7.76 +/− 0.54 |
7.14 +/− 0.47 |
8.15 +/− 0.55 |
| MIAPaCa-2 | 16.78 +/− 1.72 |
2.84 +/− 0.30 |
12.91 +/− 0.08 |
13.75 +/− 0.92 |
13.87 +/− 1.29 |
13.20 +/− 0.36 |
5.97 +/− 0.33 |
7.49 +/− 0.13 |
9.61 +/− 0.33 |
6.96 +/− 0.05 |
21.26 +/− 0.53 |
8.98 +/− 1.75 |
12.22 +/− 1.48 |
8.35 +/− 1.05 |
| Panc-1 | 18.93 +/− 0.79 |
4.93 +/− 0.98 |
11.05 +/− 1.8 |
13.29 +/− 1.19 |
13.56 +/− 1.05 |
12.95 +/− 0.84 |
4.07 +/− 0.55 |
6.50 +/− 0.36 |
10.37 +/− 0.05 |
7.64 +/− 0.01 |
20.69 +/− 0.43 |
9.27 +/− 0.23 |
10.18 +/− 0.18 |
8.09 +/− 0.26 |
| Mean Ct (Cancer cell lines) |
15.78 +/− 3.45 |
5.31 +/− 2.99 |
12.1 +/− 0.83 |
14.19 +/− 1.53 |
12.79 +/− 1.16 |
11.11 +/− 1.99 |
4.15 +/− 0.88 |
6.71 +/− 0.65 |
9.01 +/− 1.22 |
6.84 +/− 0.90 |
14.40 +/− 5.10 |
8.15 +/− 1.01 |
8.11 +/− 3.01 |
7.78 +/− 0.59 |
| HPNE | 19.80 +/− 0.12 |
3.62 +/− 0.75 | 7.93 +/− 0.31 | 5.93 +/− 1.64 | ND | ND | 6.93 +/− 1.05 | 7.70 +/− 0.87 | 8.12 +/− 0.67 | 4.78 +/− 1.33 | ND | 10.12 +/− 0.33 |
ND | 9.63 +/− 0.40 |