Figure 1.

DPSCs-CM increases the survival of wildtype and SOD1^G93A motoneurons.the number of independent experiments (each conducted in triplicate or quadruplicate) is indicated in brackets. (A) Motoneurons were immunopurified from the spinal cord of E12.5 mouse embryos and cultured in the absence (-) of a cocktail of NTFs and with increasing concentration of DPSCs-CM. Motoneurons were also cultured in the presence of NTFs (+) only. The percentage of surviving motoneurons, expressed relative to the condition without any trophic support, was calculated after 24 h. Data are means ± SEM and the number of independent experiments (each conducted in triplicate or quadruplicate) is indicated in brackets. Note that for the low concentrations of DPSCs-CM (5% and 10%), which are not relevant for motoneuron survival, the experiments were repeated twice independently, each time in triplicate. One-way ANOVA followed by Tukey’s post hoc test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s, non-significant. (B,C) Motoneurons were isolated and seeded in the absence of NTFs and with the same optimal dose previously described (50%), or only (100%) with ASCs-CM (B) or Fibro-CM (C). The number of surviving motoneurons was determined 24 h later and expressed relative to the survival in the absence of NTFs. All values are expressed as the means ± SEM of three (B) or four (C) independent experiments (triplicate or quadruplicate). One-way ANOVA followed by Tukey’s test, ** p < 0.01, n.s, non-significant. (D) Mutant SOD1^G93A motoneurons were cultured with DPSCs-CM (at 50%) or NTFs. Motoneuron survival was determined 24 h later and expressed relative to the basal condition (without NTFs). Data represent the mean ± SEM of triplicates or quadruplicates of four independent experiments.