Figure 2.

DPSCs-CM induces axon outgrowth of wildtype but not SOD1^G93A motoneurons. The number in brackets indicating the total number of motoneurons measured. (A) Representative immunostaining of a wildtype motoneuron with anti-βIII-tubulin antibody. The total axon length that also included collaterals of motoneurons (violet trace) was measured with ImageJ using the NeuronJ plugin. Scale bar = 20 µm. (B,C) Freshly purified motoneurons from E12.5 wildtype (B) or SOD1^G93A (C) embryos were treated (or not) with DPSCs-CM at the optimal concentration of 50% and NTFs. Measurement of the total axon length was performed after 24 h of culture and values are expressed relative to the basal condition (in the absence of NTFs). Graphs represent the mean value ± SEM of three independent experiments, the number in brackets indicating the total number of motoneurons measured. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. ** p < 0.01, **** p < 0.0001, and n.s, non-significant.