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. 2023 Jul 30;11(8):2152. doi: 10.3390/biomedicines11082152

Figure 4.

Figure 4

Recombinant GDF15 and HB-EGF do not provide trophic support to either wildtype or SOD1 mutant motoneurons. (A) Motoneurons were plated in basal conditions (without NTFs) and treated with indicated concentrations of recombinant GDF15 (or cultured with NTFs only). The survival of motoneurons was determined after 24 h. (B) Mouse motoneurons were treated with increasing concentrations (5, 20, and 100 ng/mL) of HB-EGF at the time of seeding. Twenty-four hours later, motoneuron survival was assessed. (C) Motoneurons immunopurified from SOD1G93A E12.5 embryos were incubated in the absence of NTFs with either GDF15 (10 ng/mL) or HB-EGF (20 ng/mL). The percentage of surviving SOD1G93A motoneurons was determined after 24h of treatment. Data represent the mean values ± SEM of triplicates of three (A,C) and four(B) independent experiments (number in brackets). Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test, *** p < 0.001, **** p < 0.0001, n.s, non-significant.