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. 2019 Dec 2;103(1):0036850419891046. doi: 10.1177/0036850419891046

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Knockdown of SIL1 inhibited the migration and invasion of human breast cancer cells. (a) Wound healing assay was used to detect the effect of SIL1 on cell migration (magnification, ×40). (b) The relative migrated surface was showed in a column diagram, which showed a significant decline in SIL1 knockdown cells in comparison with control cells. (c) The invasion capability of cells was determined using transwell assay (magnification, ×200). (d) SIL1 knockdown inhibited the invasion of MDA-MB-231 and MCF7 cells. (e) The expression level of MMP-2 was determined using gelatin zymography. (f) SIL1 knockdown inhibited MMP-2 expression according to the result of gelatin zymography.

*P < 0.05; **P < 0.01.