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. 2019 Dec 2;103(1):0036850419891046. doi: 10.1177/0036850419891046

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Knockdown of SIL1 promoted the apoptosis in human breast cancer cells. (a) The apoptosis of MDA-MB-231 and MCF7 cells was determined using flow cytometry analysis. (b) The percentage of apoptosis cell numbers was shown in a column diagram, which showed that it was enhanced by the knockdown of SIL1 both in MDA-MB-231 and MCF7 cells. (c) The expressions of apoptosis-related proteins, Bax, Bcl2, and Actived-Caspase9 were determined by western blot analysis. The expressions of Bax and Actived-Caspase9 were increased, but Bcl2 level was decreased after the transfection of si-SIL1 in MDA-MB- 231 (d) and MCF7 (e) cells.

*P < 0.05; **P < 0.01.