Figure 2.

Impact of different S. aureus isolates on biofilm formation in 96-well culture plate (Ji’s lab, unpublished data). S. aureus was incubated in TSB overnight at 37°C with shaking, then diluted at 1:200 in TSB supplemented with 3% NaCl and 0.5% glucose. The diluted bacterial cultures were transferred into the wells of human plasma-coated plate and incubated 24 h without shaking. The bacterial cultures were carefully removed, and each well was gently washed three times with PBS. The biofilm was fixed with ethanol and air dry, then stained with crystal violet solution, and washed three times with PBS. WCUH29: HA-MRSA, CFsa36: a clinical S. aureus isolate from a patient with cystic fibrosis, ST398: a LA-MRSA from a swine, 923: a CA-MRSA, JE2: a CA-MRSA. NC: no bacterial cell (negative control).