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. 2019 Nov 10;103(1):0036850419886448. doi: 10.1177/0036850419886448

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Intracellular nitric oxide (NO) level of (i) HCT 116 and (ii) HT 29 cells upon different treatments in NG and HG cultures. (a) Data are presented as relative mean fluorescence intensity normalized to the NG control (5-FU pretreated cells in NG medium free from drug substance for 48 h). (b) Representative images of DAF-FM staining cells assessed by ImageJ software (Magnification: 100 ×).

*Compared to control, p < 0.05.

**Compared to NG in the same treatment group, p < 0.05.

NG: normal glucose (5.5 mM); HG: high glucose (25 mM). L5FU: low-dose 5-fluorouracil (0.2 µg/mL); H5FU: high-dose 5-fluorouracil (2 µg/mL). LRz: low-dose rosiglitazone (0.5 µg/mL); HRz: high-dose rosiglitazone (5 µg/mL).