Skip to main content
. 2023 Aug 17;15(16):4141. doi: 10.3390/cancers15164141

Figure 8.

Figure 8

(A) HEK293 cells were transfected with the FLAG-KRAS WT or –KRASG12C; 24 h. Cells were treated with DMSO or 5 μM Sotorasib for 24 h. Cells were lysed and immunoprecipitated with FLAG antibody. IPs and cell extract were blotted with the indicated antibodies. (B) HKe-3 cells were transfected with the FLAG-KRAS WT or –KRASG12C. The cells were treated with DMSO or 5 μM Sotorasib for 24 h. Cells were lysed and immunoprecipitated with FLAG antibody. Proteins in IPs and cell extract were blotted with the indicated antibodies. (C) HEK293 cells treated as in A were blotted for the indicated antibodies. Numbers show fold changes in the level of phosphorylation of the indicated proteins. Intensities were measured using ImageJ. (D) MEF-KRAS WT and MEF- KRASG12C were treated with 5 μM Sotorasib and/or 5 μM Ruxolitinib as indicated for 24 h. Cells were lysed and the proteins were blotted with the indicated antibodies. The whole Western blot figure can be found in Supplementary Materials Original Blots and quantification for Figure 8.