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. 2023 Aug 21;15(16):4195. doi: 10.3390/cancers15164195

Figure 2.

Figure 2

Baseline expression of downstream markers of ATM and RAD51 foci scores in PDX models. (a) Representative Western blot analysis for tATM, pATM, pKAP1, and γH2AX expression in 11 PDX models. The uncropped blots are shown in File S1. (b) represents the average expression of tATM normalised to vinculin by Western blot analysis, (c) representative IHC images of pRAD50 IHC in 11 PDX models (scale bar represents 50 µm), (d) represents the mean H score of pRAD50, (e–g) represents the average expression of pATM, pKAP1, and γH2AX normalised to vinculin, respectively, and (h) represents the baseline RAD51 foci score and γH2AX staining by immunofluorescence (IF) across 9 models. Data are represented as the mean ± SEM with n = 5 for CR2506, CR3424, BN2276, GA6275, PA1221m and PA3023, n = 4, for OV2029, LU6473, and GA2254 for Western blot analysis. For RAD51 and γH2AX IHC quantification, samples that presented a low RAD51 score (<5 foci per cell) and low γH2AX score (<10% of total cells with ≥2 foci) were considered not evaluable due to the low levels of DNA damage. For detailed Western blot analysis and IF, refer to the methods section.