Table 2.
PDX model characterisation.
| Model ID | Tumour Type | Protein Change | Mutation Type | ATM VAF Original (a) |
ATM mRNA Expression [log2(Value + 1)] | tATM Mean IHC H Score |
pRAD50 Mean IHC H Score |
pATM/Vinc WB Signal (b) |
pKAP1/Vinc WB Signal (b) |
|---|---|---|---|---|---|---|---|---|---|
| CR2506 | Colorectal (ADC) | R3008H | Missense | 1 (1) | 10.28 | 22.2 | 2.45 | 0.002 (0.13) | 0.001 (1.5) |
| CR3424 | Colorectal (ADC) | K2811fs | Frameshift | 1 (0.85) | NA | 0.38 | 9.97 | 0.002 (0.11) | 0.004 (5.4) |
| OV2029 | Ovarian | R250 * | Nonsense | 1 (1) | NA | 0.05 | 1.70 | 0 (0) | 0.001 (1.9) |
| BN2276 | Glioblastoma | K2811fs | Frameshift | 0.63 (0.4) | NA | 0.05 | 19.90 | 0.64 (39) | 0.007 (10.7) |
| LU6473 | Lung | E1199 * | Nonsense | 0.53 (0.2) | 10.93 | 0.76 | 1.98 | 0.12 (6.6) | 0.019 (27.3) |
| GA2254 | Gastric | Y2514 * | Nonsense | 0.89 (0.98) | NA | 0.06 | 11.63 | 0.002 (0.11) | 0.001 (2) |
| GA6275 | Gastric (ADC) | K1773fs | Frameshift | 0.57 (0.5) | 11.33 | 0.05 | 0.64 | 0 (0) | 0.005 (7.2) |
| PA1221 | Pancreatic (ADC) | R2443 * | Nonsense | 0.97 (0.98) | 9.84 | 0.69 | 0.87 | 0 (0) | 0.001 (0.9) |
| PA3023 | Pancreatic (ADC) | N1000fs | Frameshift | 0.95 (0.97) | NA | 0.27 | 0.58 | 0 (0) | 0.011 (16) |
| LI6622 | Liver | I709I | Splice region | 1 (1) | 10.73 | 94.87 | 3.92 | 1.64 (100) | 0.069 (100) |
| CR3280 | Colorectal (ADC) | E2444K | Missense | 1 (0.98) | 10.55 | 12.68 | 2.60 | 0.016 (1) | 0.012 (16.8) |
| CTG 0828 | Large-cell lung (ADC) | E473 * | Nonsense | 1 | 1 | 2.16 | 10.6 | NA | NA |
| CTG 1140 | Head and neck (SCC) | R35 * | Nonsense | 0.73 | NA | 80.91 | NA | NA | NA |
| CTG 0166 | Lung (SCC) | W3055 * | Nonsense | 0.44 | NA | 55.49 | 10.4 | NA | NA |
| CTG 0198 | Small-cell lung | W1858 * | Nonsense | 0.42 | NA | 78.82 | NA | NA | NA |
| CTG 0149 | Head and neck | NA | NA | NA | 100 | 71.93 | NA | NA | NA |
| CTG 0776 | Head and neck | NA | NA | NA | 100 | 51.47 | NA | NA | NA |
ADC, adenocarcinoma; NA, not applicable; SCC, squamous cell carcinoma; IHC, immunohistochemistry; VAF, variant allele frequency. (a) Confirmation of ATM VAF from internal whole exome sequencing (WES) where WB refers to Western blot and (b) the % expression relative to LI6622; * truncating alterations.