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. 2023 Aug 17;12(16):2081. doi: 10.3390/cells12162081

Figure 3.

Figure 3

Myofibroblast Pfkfb3 deficiency suppresses renal fibrosis in the UUO mouse model. (A) Schematic illustration of UUO in myofibroblast Pfkfb3-deficient mice. (B) The daily body weight loss percentage for Pfkfb3f/f/PostnMCM and PostnMCM mice subjected to UUO surgery for 5 days. (C) The ratio of kidney weight to body weight for Pfkfb3f/f/PostnMCM and PostnMCM mice subjected to UUO surgery for 14 days (n = 7 PostnMCM mice, n = 6 Pfkfb3f/f/PostnMCM mice). (D) The ratio of fibrotic kidney weight to control kidney weight for Pfkfb3f/f/PostnMCM and PostnMCM mice subjected to UUO surgery for 14 days (n = 6 mice per group). (E) qRT-PCR analysis of the mRNA expression of Acta2, Col1, Col3, Mmp2, and Mmp9 in kidneys collected from Pfkfb3f/f/PostnMCM and PostnMCM mice at day 14 post-UUO surgery (n = 8 mice/group). (F) Representative Western blots and their quantification showing ACTA2, Vimentin, COL I, and FN protein expression in kidneys collected from Pfkfb3f/f/PostnMCM and PostnMCM mice at day 14 post-UUO surgery (n = 7 PostnMCM mice, n = 6 Pfkfb3f/f/PostnMCM mice). Data are means ± SEM. ns, no significance; * p < 0.05; ** p < 0.01; *** p < 0.001 for indicated comparisons. Statistical significance was determined using one-way ANOVA followed by the Bonferroni test (C,E) or using Student’s t-test (D,F).