Table 4.
Two-way ANOVA for FosB and JunB in the cerebellum of wild-type, TLR4-, MyD88-, and TRIF-deficient mice. GraphPad Prism 9 was utilized to perform ordinary two-way analysis of variance (ANOVA) as described in the Methods. The main effects of treatment (control or ethanol) and genotype (wild-type or TLR4_KO, MyD88_KO, or TRIF_KO) and their interaction were evaluated. p values < 0.05 were considered significant. *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant.
| FosB | Wild-Type vs. TLR4_KO | Wild-Type vs. MyD88_KO | Wild-Type vs. TRIF_KO | ||||||
| Two-Way ANOVA | F (DFn, DFd) | p Value | Sig. | F (DFn, DFd) | p Value | Sig. | F (DFn, DFd) | p Value | Sig. |
| Interaction | F (1, 48) = 8.583 | 0.005 | ** | F (1, 49) = 9.414 | 0.004 | ** | F (1, 45) = 2.203 | 0.14 | ns |
| Genotype | F (1, 48) = 8.584 | 0.005 | ** | F (1, 49) = 9.421 | 0.003 | ** | F (1, 45) = 2.204 | 0.14 | ns |
| Treatment | F (1, 48) = 30.17 | <0.001 | *** | F (1, 49) = 32.22 | <0.001 | *** | F (1, 45) = 31.07 | <0.001 | *** |
| JunB | Wild-type vs. TLR4_KO | Wild-type vs. MyD88_KO | Wild-type vs. TRIF_KO | ||||||
| Two-way ANOVA | F (DFn, DFd) | p value | Sig. | F (DFn, DFd) | p value | Sig. | F (DFn, DFd) | p value | Sig. |
| Interaction | F (1, 48) = 8.811 | 0.005 | ** | F (1, 51) = 4.386 | 0.04 | * | F (1, 46) = 0.8740 | 0.35 | ns |
| Genotype | F (1, 48) = 8.824 | 0.005 | ** | F (1, 51) = 4.327 | 0.04 | * | F (1, 46) = 0.8699 | 0.36 | ns |
| Treatment | F (1, 48) = 18.09 | <0.001 | *** | F (1, 51) = 32.12 | <0.001 | *** | F (1, 46) = 25.82 | <0.001 | *** |