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. 2023 Aug 15;12(16):2069. doi: 10.3390/cells12162069

Figure 2.

Figure 2

1-Heptanol enhances the lateral mobility of FcεRI, as determined by FRAP, but retards internalization. (A) IgE-FITC-sensitized RBL-2H3 cells were exposed for 10 min to the vehicle (BSS-BSA; Control) or 2.5 mM 1-heptanol. Representative fluorescence image sequences are from cells at the indicated time points before photobleaching (Pre-bleach), at photobleaching (Bleach; the circled areas) or after photobleaching (Post-bleach; the circled areas). Bars indicate 10 µm. (B) Quantification of the recovery into the photobleached areas as shown in A. The relative fluorescence intensity was calculated at each time point by dividing the fluorescence in the bleached region by the fluorescence in the corresponding unbleached region. The background was subtracted from all signals. Values in (B) indicate means ± SEM of two independent experiments for the control cells (n = 16) and 1-heptanol-treated cells (n = 15). (C) FcεRI internalization in antigen-activated BMMCs in the absence (control, n = 5) or presence of 2.5 mM 1-heptanol (n = 5). Statistical significance of intergroup differences is indicated in (B,C); * p < 0.05, ** p < 0.01.